Energy transduction in Propionibacterium acnes

Noor, M. A. Mohd.

Biochemistry
February 1983

Thesis or dissertation


Rights
© 1983 M A Mohd Noor. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright holder.
Abstract

1. Propionibacterium acnes was shown to be sensitive to valinomycin thus enabling the membrane potential (∆Ψ) generated under a variety of conditions to be measured by determining the distribution of K⁺ across the membrane in the presence of valinomycin.
2. Under anaerobic conditions, both anaerobically- and aerobically-grown P. acnes generated a ∆Ψ of approximately -140 mV. Oxygen stimulated the generation of the ∆Ψ in both anaerobically- and aerobically-grown cells. The effect of inhibitors of oxidative phosphorylation on the ∆Ψ generated was studied.
3. Anaerobically- and aerobically-grown P. acnes were capable of uncoupler-stimulated respiration. The effect of various inhibitors on respiration was studied.
4. Respiration-driven H⁺ translocation was demonstrated in both anaerobically- and aerobically-grown P. acnes. The →H⁺/O ratios obtained were consistent with the presence of one coupling site on the electron transport chain. The effect of inhibitors on respiration-driven H⁺ translocation was studied.
5. Anaerobically- and aerobically-grown P. acnes contained cytochromes b, a₁, d and o.
6. Attempts to characterize the ATPase from P. acnes and to demonstrate its sensitivity to classical energy-transfer inhibitors were unsuccessful.
7. Using the results obtained from the various approaches, a role for oxygen in the energy transduction process of P. acnes was proposed.

Publisher
Department of Biochemistry, The University of Hull
Supervisor
Midgley, Melvin
Sponsor (Organisation)
Universiti Sains Malaysia
Ethos identifier
uk.bl.ethos.353756
Qualification level
Doctoral
Qualification name
PhD
Language
English
Extent
4 MB
Identifier
hull:13778
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